Brad Walker gave Hartlepool United a boost by passing a fitness test on an ankle injury.
Walker, outstanding since his conversion from midfield into defence, missed Saturday's defeat at Notts County.
He returned to partner Scott Harrison, with Lewis Hawkins dropping down to the bench.
Cambridge boss Shaun Derry made two changes to the side which won 3-0 at promotion hopefuls Carlisle on Saturday, including the inclusion of former Pools left-back, Jake Carroll.
Carroll and Harrison Dunk came ion for Greg Taylor and George Maris, but there was no place for Brunton Park supersub, Medy Elito, who was named on the bench despite bagging a hat-trick.
Cambridge: Norris, Wharton, Halliday, Legge, Dunk, Mingoia, Dunne, Clark, Berry, Carroll, McDonagh.
Subs: Roberts, Williamson, Taylor, Gregory, Elito, Maris, Lewis
Pools: Fryer, Richardson, Walker, Harrison, Donnelly, Featherstone, Woods, Alessandra, Thomas, Oates, Amond.
Subs: Bartlett, Martin, Hawkes, Hawkins, Deverdics, Orrell, Rooney.
Referee: Dean Whitestone